Memory deficient mouse created by eliminating kinase activity of CaMKIIalpha
Knock-in mouse created that shows memory deficits by eliminating kinase activity of CaMKIIalpha. Brain and memory research shows the memory deficient mouse exhibited impaired tetanus-induced long term potentiation (LTP) and sustained postsynaptic spine enlargement. These impairments are believed to be related to the mutant mouse deficit in inhibitory avoidance learning.
June 19, 2009
Ca2+/calmodulin-dependent protein kinase II alpha (CaMKII alpha) is an enzyme that adds phosphates to a variety of protein substrates to modify their functions. CaMKII alpha is enriched in the hippocampus, the memory center of the brain, and is believed to be an essential mediator of activity-dependent synaptic plasticity and memory functions. However, the causative role of the enzymatic activity of CaMKII alpha in such processes has not been demonstrated yet, because this enzyme has multiple protein functions other than the kinase activity. A Japanese research group, led by Dr Yoko Yamagata of the National Institute for Physiological Sciences, Japan, has successfully generated a novel kinase-dead mutant mouse of the CaMKII alpha gene that completely and exclusively lacks its kinase activity. They examined hippocampal synaptic plasticity and behavioral learning of the mouse, and found a severe deficit in both processes. They reported their findings in the Journal of Neuroscience, published on June 10, 2009.
The research group successfully generated a novel CaMKII alpha (K42R) knock-in mouse that completely lacks the kinase activity of CaMKII alpha, and examined the effects on structural, functional, and behavioral expression of synaptic memory. In the K42R brain, tetanus-induced long-term potentiation (LTP), a proposed cellular mechanism of memory, and sustained postsynaptic spine enlargement, a structural basis for LTP, were both impaired, whereas dynamic postsynaptic movement of CaMKII alpha protein was preserved. In addition, the K42R mouse showed a severe deficit in inhibitory avoidance learning, a form of memory dependent on the hippocampus. The research group concluded that the mutant mouse could not form memories and did not remember the events that had just happened.
Neuroscience Memory Research Article Details:
This research was carried out in collaboration with Profs. Shigeo Okabe, Toshiya Manabe, Yuchio Yanagawa, Keiji Imoto, Kunihiko Obata and others. Dr Yoko Yamagata and Prof Yuchio Yanagawa have applied for a patent on this technology through Japan Science and Technology Agency (JST).
Source: National Institute for Physiological Sciences
Contact: Dr. Yoko Yamagata National Institute of Physiological Sciences